• Our xeno free culture conditions made single cell

  2018-10-22

  

  Our xeno-free culture conditions made single cell passaging possible, and we could also generate EBs from single cell suspensions. Although both hESC lines retained a normal karyotype, we did observe an accumulation of a chromosomal abnormality in one of our cultures. After 13 passages under xeno-fr

• One approach to generate specific cell populations is to dir

  2018-10-22

  

  One approach to generate specific cell populations is to direct PSCs toward a desired cell type by sequential progression through intermediate cell populations, mimicking the stages of embryonic development. Monolayer differentiation approaches allow homogeneous accessibility of Cy3.5 hydrazide to

• br Experimental Procedures br Acknowledgments This work was

  2018-10-22

  

  Experimental Procedures Acknowledgments This work was supported by BONFOR/Gerok Scholarships O-137.0015 (to B.V.S.), Ruediger Foundation grants (to B.V.S.), State Scholarship Fund/Chinese Scholarship Council 2008627116 (to Z.L.), NIH – National Eye Institute 5R01EY022079-02 (to S.T.), and NYSD

• Last but not least the differentiation

  2018-10-22

  

  Last, but not least, the differentiation method described here can be refined to be fully defined and xeno-free by substituting human serum albumin (HSA) present in RegES− medium with recombinant HSA (He et al., 2011) and using a higher certificate, xeno-free version of CnT-30 medium (CELLnTEC Advan

• Fibroblasts can be directly reprogrammed into neurons Vierbu

  2018-10-22

  

  Fibroblasts can be directly reprogrammed into neurons (Vierbuchen et al., 2010), cardiomyocytes (Ieda et al., 2010), and NSCs (Han et al., 2012) using tissue-specific combinations of transcription factors. However, the pluripotent reprogramming cocktail of Oct4, Sox2, Klf4, and Myc can also convert

• The recovery of ExEn outer layer

  2018-10-20

  

  The recovery of ExEn outer layer and down-regulation of pluripotent genes in mutant EBs grown in medium supplemented with BMP4 indicate that the failure of Pelo-null cells to down-regulate pluripotency-related genes in EBs is not a result of cell-autonomous effect, but rather to failed signals from

• Taken together we conclude that Lats

  2018-10-20

  

  Taken together, we conclude that Lats may regulate adult cardiomyocyte hypertrophy independent of Yap or Tead1. Rather than controlling cardiac hypertrophic growth, the canonical Hippo/Yap pathway is crucial for regulation of cardiomyocyte survival in the adult heart. This parallels the findings in

• br Materials and methods br Results

  2018-10-20

  

  Materials and methods Results Discussion riPSCs are typically generated and cultured on MEF feeders (Liu et al., 2008). Feeders are sufficient to support rhesus iPSC long-term maintenance. However, this approach is too labor intensive for large-scale culture. Optimization of this culture sy

• br Materials and methods br Results br

  2018-10-20

  

  Materials and methods Results Discussion Obesity results when caloric intake exceeds energy expenditure, with an increase in adipocyte size or adipocyte number (Bjorntorp, 1974; Faust et al., 1978; Johnson et al., 1971, 1978; Yu et al., 1997). In the context of adipogenesis, development app

• In this study we demonstrated that unrelated

  2018-10-20

  

  In this study, we demonstrated that unrelated allogeneic human MSCs supported a LSC phenotype in a largely quiescent state in a long-term vitro culture system. We then confirmed the persistence of LIC after 6weeks co-culture with MSCs using the NSG mouse xenotransplant model. Our results also reveal

• br Source of funding This work was

  2018-10-20

  

  Source of funding This work was supported by research funding from The Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Scientific Research (C) (22591555) and the Riken Center for Developmental Biology Collaborative Research Fund, Kobe (08001475). Disclosures

• Currently because of the lack

  2018-10-20

  

  Currently, because of the lack of biomarkers that identify DSCs specifically, the only way to monitor relative changes in their number and quality is by functional assays. In animal model research, changes in DSCs are monitored by the ability of transplanted donor corticotropin releasing factor to

• UV induced changes in the cornea are largely mediated

  2018-10-20

  

  UV-induced changes in the cornea are largely mediated by the up-regulation of pro-inflammatory cytokines such as interleukin (IL)1 (Corsini et al., 1997), IL6 (Di Girolamo et al., 2002), IL8 (Di Girolamo et al., 2002) and tumour necrosis factor alpha (TNFα) (Kennedy et al., 1997) which correlate to

• Following from our prior research

  2018-10-20

  

  Following from our prior research to derive new hESC lines under increasingly defined culture conditions (Fletcher et al., 2006) we sought to establish a facility within which our experience and evolving practice and reagents could be implemented to a GMP and professionally accredited standard. Here

• br Materials and methods br Results br Discussion The role

  2018-10-20

  

  Materials and methods Results Discussion The role played by Nurr1 in the regulation of NPCs across several beta lactamase inhibitor regions has been extensively investigated. It has been suggested that Nurr1 may exert a strong control on both the proliferation and differentiation of NPCs in

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