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For cCFU F analysis of
2018-11-12
For cCFU-F, analysis of differential gene expression implied a possible ‘memory of tissue origin’. The gene encoding the MADS box transcription factor, Mef2c, essential for deployment of cardiac progenitor cells to the forming heart (Lin et al., 1997), was cCFU-F specific and expressed 6.6-fold and
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br Long term maintenance of
2018-11-12
Long term maintenance of hPSCs in a scalable and defined suspension culture system A report published recently in Stem Cell Research (Chen et al., 2012, 8:388–402) extends from these previous studies to demonstrate for the first time, the long term expansion of hESCs retaining the characteristic
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Currently there is limited information on the stability of t
2018-11-12
Currently, there is limited information on the stability of the mitochondrial genome during expansion of adult cells. A very limited number of studies have reported the detection of mutations in the mtDNA of human colonic crypt stem purchase PJ 34 as well as in adult and embryonic primate stem cell
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Unfortunately existing cytogenetic karyotyping methods are l
2018-11-12
Unfortunately, existing cytogenetic karyotyping methods are labor intensive, requiring at least 15 fixed metaphase preparations, subsequent DAPI staining, and analysis with an epi-fluorescence microscope equipped with specialized digital imaging software. Conventional karyotyping is expensive (∼$400
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br Experimental Procedures br Acknowledgments This work
2018-11-12
Experimental Procedures Acknowledgments This work was supported by funding from buy GSK J4 ICR. F.B.A. was supported by the Erasmus Work Placement Programme. We thank Drs. Taiping Chen (MD Anderson Cancer Center) for providing J1 WT and Dnmt1-null ESCs and Masaki Okano (Riken) for providing TK
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pgds inhibitor Introduction The vascular endothelium the sin
2018-11-12
Introduction The vascular endothelium, the single-cell layer lining blood vessels, is a multifunctional interface that displays a striking phenotypic plasticity necessary for maintaining vascular homeostasis. In this context, the vascular endothelium is critical to initiate an inflammatory response
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Several transcripts that exhibited an expression change trig
2018-11-12
Several transcripts that exhibited an expression change triggered by T3 are promising candidates for being responsible for the observed increased cell extension growth of ENS cells in vitro. The calcium dependent cell adhesion molecule cadherin 12 (Cdh12, N-cadherin, 5.6 fold upregulated), the gene
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br Materials and methods br Results There are a number
2018-11-12
Materials and methods Results There are a number of complex methods for the generation of neural subtypes from hESCs and iPSCs. In most of them, intact pluripotent colonies are first lifted, induced to form EBs, and then grown in the presence of lineage specific morphogens and growth factors t
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histone deacetylase inhibitors DNA fragmentation is an essen
2018-11-12
DNA fragmentation is an essential component of PCD. Of several nucleases that were tested for their involvement in charontosis, EndoG emerged as a likely candidate, although a role for CAD cannot be discounted due to the degradation of ICAD observed 44 h after ETO treatment. EndoG is activated in hi
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their mathematical expectations follow the expressions
2018-11-09
their mathematical expectations follow the expressions According to the CLT for independent random variables , for N → ∞ the expression tends to a standardized Gaussian random variable. Therefore for N ≫ 1 we have the following approximate equalities: where θ0, , are the standardized Gaussian
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br It is reasonable to relate
2018-11-09
It is reasonable to relate the conditional expectation of the deviation for the regression (2) at the given Ro 3306 and source, namely, to the systematic error of the source, e.g., the procedure error of the method for obtaining the cross-sections for each energy. In this case the conditional exp
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br Discussion The findings from our
2018-11-09
Discussion The findings from our study were generally consistent with previous research that showed a significant association between neighborhood social brain metabolism and physical activity. Specifically, several studies have shown that high neighborhood social cohesion is associated with gre
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br Ethical approval br Introduction The presence of
2018-11-09
Ethical approval Introduction The presence of metal ions in the environment is of major concern due to their toxicity to many life forms. Unlike organic pollutants, the majority of which are susceptible to biological degradation, metal ions do not degrade into harmless end-products. The metals
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In this study we examined the role of ADAM in
2018-11-09
In this study, we examined the role of ADAM17 in colitis by developing dextran sulfate sodium (DSS)-induced colitis using two different conditional Adam17-deficient mice, i.e. mice with systemic deletion by the inducible Mx1-Cre gene of Adam17 (Adam17Mx1-Cre+) or those with its deletion in myeloid M
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br Introduction Today about of treated
2018-11-09
Introduction Today, about 90% of treated persons living with HIV are aviremic (Delaugerre et al., 2015). Yet, 6 to 24% of these virologic responders do not restore their CD4 count as they should, exposing them to the risk of immune deficiency-linked morbidities (Corbeau and Reynes, 2011). The mai
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